Dihydroartemisinin is a newer approach to the treatment of Malaria. Here, DHA has no strong UV absorbing groups and it is optically alive. It converts from α-DHA to β-DHA in organic solvents which can be estimated by RP-HPLC. The DHA was determined by RP-HPLC method by using Kromasil C18 (250 mm x 4.6 mm i.d., 5 µm particle size) column. A mobile phase composed of acetonitrile, methanol and water in proportion of 60:20:20 v/v, at flow rate of 0.8 ml/min was used for the separation. Detection was carried out at 215 nm. Method was validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in pharmaceutical formulations with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.
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